03 April 1980

Research - 14 Years (including 5 years at MIT, USA and 2 years at SFU, Canada)
Teaching - 6 Months

Biotechnology/Microbiology/ Immunology/ Protein Science and Engineering/Enzymology/Microbial genetics/gene expression regulation.

Microbiology, Biochemistry, Molecular Biology, Genetic Engineering, Biotechnology, Bioinformatics, Environmental Science and other related subjects.

(A) Biochemical characterization of pathogenic mutations in a human mitochondrial enzyme called methionyl-tRNA formyltranferase.
(B) Isolation of suppressor mutants in an Escherichia coli protein kno

• Qualified all India National Eligibility Test (CSIR-NET) for Junior Research Fellowship twice in 2004.
• Qualified all India ICMR-JRF test for Junior Research Fellowship in 2004.
• Scored 98.2 percentile in the Graduate Aptitude Test in Engineering (GATE) in 2004.
• Qualified all India Combined Entrance Examination conducted by JNU for admission to M. Sc. Biotechnology Programme in 2002.
• First class first rank (Gold medalist) in B. Sc Biotechnology from Ranchi University in 2002.

Awarded Junior reserach and senior reserach fellowship award from CSIR.

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• Sinha, A., Kohrer, C., Weber, M. H., Masuda, I., Mootha, V. K., Hou, Y. M. and RajBhandary, U. L. (2014) Biochemical characterization of pathogenic mutations in human mitochondrial methionyl-tRNA formyltranferase. J Biol Chem 289, 32729-32741.

• Pathak, A., Goyal, R., Sinha, A. and Sarkar, D. (2010) Domain structure of virulence associated response regulator PhoP of Mycobacterium tuberculosis: Role of linker region on regulator-promoter interactions. J Biol Chem 285, 34309-34318.

• Das, A., Pathak, A., Sinha, A., Datt, M., Singh, B., Karthikeyan, S. and Sarkar, D. (2010) A single amino acid substitution in the C terminus of PhoP determines DNA binding specificity of the virulence-associated response regulator from Mycobacterium tuberculosis. J Mol Biol 398, 647-656.

• Gupta, S., Pathak, A., Sinha, A. and Sarkar, D. (2009) Mycobacterium tuberculosis PhoP recognizes two adjacent direct repeat sequences to form head-to-head dimers. J Bacteriol 191, 7466-7476.

• Sinha, A., Gupta, S., Bhutani, S., Pathak, A. and Sarkar, D. (2008) PhoP-PhoP interaction at adjacent PhoP binding sites is influenced by protein phosphorylation. J Bacteriol 190, 1317-1328.

• *Gupta, S., *Sinha, A. and Sarkar, D. (2006) Transcriptional autoregulation by Mycobacterium tuberculosis PhoP involves recognition of novel direct repeat sequences in the regulatory region of the promoter. FEBS Letters 580, 5328-5338 (* contributed equally to the manuscript).

Enthusiam, inquisitveness and commitment for acquiring knowledge through study and research.

Need to work on multi tasking abilities.